Ηematologic Μalignancies

Immunophenotype, histological & genetic approach to hematological diseases.

Immunophenotype tests of peripheral blood and marrow
Classical Cytogenetic Analysis of Peripheral Blood and Bone Marrow (Karyotype).
Molecular Cytogenetic Analysis (FISH).
Molecular tests of hematological neoplasias.
Histological examinations of osteomyelic biopsy material, lymph nodes and study of extra-lymphatic lymphomas.

Flow cytometry

Immunophenotypic analysis standardization of subpopulations of peripheral blood lymphocytes, T, B, NK. Control B and T clonality
Bone marrow immunophenotype (investigation of myelodysplastic syndromes, myeloproliferative neoplasms, investigation of multiple myeloma, leukemia standardization, etc.)
Standardization of Lymphoproliferative Diseases.
Detection of clone PNH (screening for the presence of paroxysmal night hemoglobinuria)

Molecular cytogenetic analysis

Chronic myeloid leukemia

Acute myeloid leukemia

Detection of Trisomy Chromosome 13
Rearrangement Detection of the MLL Gene (11q23)
Detection of Chromosomal Permutation t(1;22)(p13;q23)
Trisomy Chromosome 21 Detection
Detection of Chromosome Trisomy 8
Chromosome Inversion Detection 16 (CBFb/MYH11)
Detection of Monosomy/ Lack of Chromosome 5 (-5/5q-)
Detection of Monosomy/ Chromosome 7 Deficiency (-7/7q-)
Detection of Chromosomal Permutation t(15;17)(q22;q11-21)
Detection of Chromosomal Permutation t(8;21)(q22;q22)
Detection of Inversion or Permutation of the EVI I Gene [inv(3)(q21q26), t(3;3)(q21;q26)](M1, M2, M4, M6, M7)
Deficiency Detection on Chromosome 20 (del 20q)

Acute lymphoblastic leukemia

Detection of Chromosomal Permutation t(12;21)(p13;q22) (TEL/AML1)
Rearrangement Detection of the MLL Gene (11q23)
Detection of Chromosomal Permutation t(9;22)(q34;q11) (BCR/ABL)
Detection of Chromosomal Permutation t(1;19)(q23;p13)
Trisomy Chromosome 21 Detection
Deficiency Detection on Chromosome 9 del(9p21)
Detection of Chromosomal Permutation t(8;14)(q24;q32)
C-MYC Gene Rearrangement Detection (8q24)
Rearrangement Detection of the E2A Gene (19p13)

Myelodysplastic syndromes

Detection of Chromosome Trisomy 8
Trisomy Chromosome 21 Detection
Chromosome 16 REVERSE DETECTION (CBFb/MYH11)
Detection of Monosomy/ Lack of Chromosome 5 (-5/5q-)
Detection of Monosomy/ Chromosome 7 Deficiency (-7/7q-)
Deficiency Detection on Chromosome 20 (del 20q)
Detection of Inversion or Permutation of the EVI I Gene [inv(3)(q21q26), t(3;3)(q21;q26)](M1, M2, M4, M6, M7)
Y Chromosome Deficiency Detection

Multiple myeloma

Lymphomata

Detection of Chromosomal Permutation t(2p23) (ALK)
Detection of Chromosomal Permutation t(8q24) t(8;22)(q24;q11) and t(2;8)(p11;q24)
Detection of Chromosomal Permutation t(8;14)(q24;q32)
Detection of Chromosomal Permutation t(11;14) (q13;q32) (BCL1/IgH)
Detection of Chromosome Trisomy 12
IGH Gene Rearrangement Detection (14q32)
Chimerism (after allogeneic transplantation)
XX/XY FISH chimerism detection

Chronic lymphoproliferative syndromes

Deficiency Detection on Chromosome 11 (11q22.3)
Detection of Chromosome Trisomy 12
Deficiency Detection on Chromosome 13 (13q34)
Deficiency Detection on Chromosome 13 (13q14.3)
Deficiency Detection on Chromosome 13 (13q14)
Deficiency Detection on Chromosome 17 (17p13.1)
t(14;18) permutation detection(q32;q21)
IGH Gene Rearrangement Detection (14q32)
Permutation detection t(11;14)(q13,q32)
Deficiency Detection on Chromosome 6 (6q23.3)

Chronic myeloproliferative syndromes

MOLECULAR PATHOLOGY & GENETICS